Screening of Process Parameters to Produce Xylanase from Aspergillus niger for Secondary Bioethanol Production

<jats:title>Abstract</jats:title>
<jats:p>In recent years, the biotechnological use of xylanases has grown remarkably. Xylanase is a hydrolytic enzyme with a broad industrial application. In specific, xylanase can convert xylan into xylose, a fermentable sugar source for secondary bioethanol production. The objective on this study is to investigate the significance of different parameter effects for an efficient xylanase production from <jats:italic>Aspergillus niger (A. niger).</jats:italic> In this study, four factors: incubation temperature, medium pH, incubation time, and agitation speed were screened by performing One-factor-at-a-time (OFAT) analysis. Xylanase production with the maximal enzyme activity was successfully obtained from OFAT analysis under condition of 32°C, pH 5.0, 5 days, and 150 rpm.</jats:p>