Lovy PerdaniNudia Mufidah AzasiFajar SofyantoroRudy Agung NugrohoNor Ismaliza Binti Mohd IsmailWoro Anindito Sri Tunjung2026-10-162026-10-162025-03-0310.12991/jrespharm.1644627https://dspace-cris.utar.edu.my/handle/123456789/11768This study examined the anti-aging effect of Chinese perfume (Aglaia odorata) and Indian camphorweed (Pluchea indica) leaf extracts on Saccharomyces cerevisiae as a model system. Investigation of the antioxidant activity and bioactive compounds using GC–MS and qualitative anti-aging spot tests were performed to determine the anti-aging effects. In addition, a quantitative anti-aging test was conducted using high-throughput chronological lifespan analysis. The results showed that the antioxidant enzyme activities of SOD, APX, and CAT in Chinese perfume leaves were 393.96 units/min/g FW, 215 µmoles H2O2/min/g FW, and 5.6 µmoles H2O2 decomposed/min/g FW, respectively; the values in Indian camphorweed leaves were 717.57 units/min/g FW, 48 µmoles H2O2/min/g FW, and 12.33 µmole H2O2 decomposed/min/g FW, respectively. The antioxidant activity of Chinese perfume and Indian camphorweed was 577.2 µg/mL and 348.86 µg/mL. The antioxidant bioactive compounds of Chinese perfume extract included n-hexadecanoic acid, ? turmerone, and 2-propenoic acid, 3-phenyl-, methyl ester (methyl cinnamate) and those from Indian camphorweed included n-hexadecanoic acid and neophytadiene. Treatment with both extracts prolonged the life of yeast after 15 days of incubation. In addition, H2O2 stress conditions, the yeasts showed better growth with the addition of both leaf extracts. This study revealed that the extracts of Chinese perfume and Indian camphorweed leaves demonstrate promising potential as ingredients for anti-aging cosmetics. © 2025 Marmara University Press.en-USanti-agingantioxidantChinese perfumeIndian camphorweedS. cerevisiaeacetic acid ethyl esteracrylic acidalcoholascorbate peroxidaseascorbic acidcatalasecinnamic acidcosmetichydrogen peroxidepalmitic acidpalmitic acid methyl esterperfumeplant extractsuperoxide dismutaseturmeroneagingAglaiaantiaging activityantioxidant activityArticlecell viabilitycontrolled studyenzyme activityIndonesialifespanmodelnonhumanphysiological stressplant leafPluchea indicaquantitative analysisSaccharomyces cerevisiaeyeastjournal-article