Siti Nur Syahida Abdul JalilNg Chin Theng0000-0001-7912-6746Lai Yen FONGMuhammad Nazrul HakimZuraini Ahmad2026-10-192026-10-192026-03-2010.13005/bpj/3391https://dspace-cris.utar.edu.my/handle/123456789/11782Inflammation is a protective physiological response that may cause tissue damage when prolonged. Copper–amino acid complexes, including Cu(L-alanine)2, have been reported to possess various biological activities. However, their anti-inflammatory potential remains underexplored. This study evaluated the in vivo anti-inflammatory effects of Cu(L-ala)2 using three experimental models in rats: acetic acid-induced peritoneal vascular permeability, carrageenan-induced paw edema, and nitric oxide (NO) production in paw tissue. Rats were divided into four groups (n = 6) and treated with vehicle control (1% DMSO), indomethacin (10 mg/kg), or Cu(L-ala)2 at doses of 2 or 20 mg/kg. Acetic acid markedly increased vascular permeability, which was significantly inhibited by Cu(L-ala)2 by 74.1% (2 mg/kg) and 81.23% (20 mg/kg). In the paw edema model, both doses significantly reduced inflammation compared with the control, with the 2 mg/kg dose showing the greatest inhibition (63.79%) at 1 hour. In contrast, Cu(L-ala)2 produced only a moderate reduction in NO levels, reaching statistical significance only at 20 mg/kg (27.56%), while indomethacin showed a stronger effect (46.28%). Overall, Cu(L-ala)2 demonstrated significant anti-inflammatory activity by reducing vascular permeability and edema formation. However, its limited effect on NO production suggests that its mechanism of action may be largely NO-independent. Further studies are required to clarify its molecular targets, dose–response behavior, and therapeutic potential. Oriental Scientific Publishing Company © 2026.en-USAnimal modelsAnti-inflammatoryCu(L-Alanine)2Paw edemaPeritoneal vascular permeabilityalaninecopper l alanineindometacinnitric oxidenonsteroid antiinflammatory agentunclassified druganimal experimentanimal modelantiinflammatory activityArticlebiological activityblood vessel permeabilitycarrageenan-induced paw edemacontrolled studyinflammationmalenonhumanratjournal-article