Zhuo-Yu CaiZheng-Yang NiuYou-Yuan ZhangYi-Hua TongTien Chinh VuWei Lim GohSarawood SungkaewAtchara TeerawatananonNian-He Xia2024-10-162024-10-162023-12-04https://doi.org/10.3389/fpls.2023.1274337https://dspace-cris.utar.edu.my/handle/123456789/2730<jats:p><jats:italic>Neomicrocalamus</jats:italic> and <jats:italic>Temochloa</jats:italic> are closely related to bamboo genera. However, when considered with newly discovered and morphologically similar material from China and Vietnam, the phylogenetic relationship among these three groups was ambiguous in the analyses based on DNA regions. Here, as a means of investigating the relationships among the three bamboo groups and exploring potential sources of genomic conflicts, we present a phylogenomic examination based on the whole plastome, single-nucleotide polymorphism (SNP), and single-copy nuclear (SCN) gene datasets. Three different phylogenetic hypotheses were found. The inconsistency is attributed to the combination of incomplete lineage sorting and introgression. The origin of newly discovered bamboos is from introgressive hybridization between <jats:italic>Temochloa liliana</jats:italic> (which contributed 80.7% of the genome) and <jats:italic>Neomicrocalamus prainii</jats:italic> (19.3%), indicating that the newly discovered bamboos are closer to <jats:italic>T. liliana</jats:italic> in genetics. The more similar morphology and closer distribution elevation also imply a closer relationship between <jats:italic>Temochloa</jats:italic> and newly discovered bamboos.</jats:p>journal-article