K.GobiVel M. Vadivelu2024-10-302024-10-302017-04-1810.1002/clen.201600314https://dspace-cris.utar.edu.my/handle/123456789/5609The effect of triglyceride on polyhydroxyalkanoate (PHA) accumulation in aerobic granules used for treating palm oil mill effluent (POME) is unaddressed to date. Triglyceride was added (between 3.7 and 18.5 g/L) to a batch reactor containing aerobic granules and POME. The chemical oxygen demand (COD) removal, PHA content, and PHA composition were analyzed at different triglyceride concentrations. The COD removal decreased to 19% when 18.5 g/L triglyceride was added. Meanwhile, the PHA content decreased from 0.62 to 0.16 g PHA/g cell dry weight (CDW) as the triglyceride concentration increased from 0 to 18.5 g/L. The composition of PHA did not change significantly with the increase in triglyceride concentrations. Accumulated PHA was composed of hydroxybutyrate (HB) and hydroxyvalerate (HV) with a ratio of approximately 77:23. Eventually, the triglyceride was found to limit oxygen uptake, carbon metabolism, and PHA accumulation, in aerobic granules.journal-article